Treatment with Gaseous Ozone Significantly Reduced the Number of Bacteria in Extended-Spectrum-β-Lactamase (ESBL)-Producing Escherichia coli Biofilm

Ozone is a triatomic allotropic modification of oxygen with very high oxidation potential and strong antimicrobial properties, can be used as disinfecting agent. The aim this work was to investigate the effectiveness gaseous ozone in reducing number bacteria total biomass E. coli biofilm using different methods quantification detection. Biofilm all tested clinical isolates standard strain grown on ceramic tiles dimensions 1.0 × cm over 24 h. These plates were then treated for 1 After washing, CFU/cm2 determined, ATP bioluminescence measured luminometer, reduction after crystal-violet staining. Gaseous proved effective destroying created bacterial tiles. Treatment caused up 2.00 log10CFU/cm2, followed by 61.40%. Inhibition rates varied from 35.80% 99.41%, depending method used. All study are determining anti-biofilm activity ozone, but more research needed.